Table S3 | Ka/Ks analysis of BZR gene pairs in five Rosaceae genomes. (2012). cv 'Yali') fruit and an analysis of the expression of the gene. PloS One 9, e92644. Chromosomal level reference genomes provide a crucial foundation for genomics research such as genome-wide association studies (GWAS) and whole genome selection. Interspecific hybrid species of pear native to North China, where it is widely grown for its edible fruit. doi: 10.1093/bioinformatics/btp187, Liu, L., Jia, C., Zhang, M., Chen, D., Chen, S., Guo, R., et al. For copyright and licence information, see the Licence page. Pyrus bretschneideri Rehder, Proc. MYB transcription factors in chinese pear (Pyrus bretschneideri Rehd. In general, Ka/Ks < 1 indicates functional constrain with the negative/purifying selection, Ka/Ks > 1 suggests accelerated evolution with the positive selection, and Ka/Ks = 1 indicates the genes are drifting neutrally (Yang, 2007). doi: 10.1093/nar/gkr931, Li, J., Chory, J. Biol. A member of the Rosaceae family, Pyrus bretschneideri Rehder does not go by a known (to us) common name. Expression of fifteen lignin synthetic genes (PbPAL2, PbC4H1, PbC4H3,Pb4CL12,PbHCT6, PbC3H1, PbCES9, PbCCoAMOT1, PbCCoAOMT2, PbF5H1, PbF5H3, PbCOMT3, PbCCR20, PbCAD1, and PbCAD2) was increased in PbBZR1-RNAi fruits (Figure S7), which was consistent with the increase of lignin content in the fruits of silencing (Cao et al., 2019). U. These results implied that both exon loss and gain has occurred during the long evolutionary period of the BZR gene family, which further may explain the functional differences of closely related-BZR gene family members. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. Acad. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Amer. To further understand the role of PbBZR1/2/3, we used a VIGS (virus-induced gene silencing) to suppress the expression of PbBZR1/2/3 in P. Bretschneideri fruit (Figure 6A). Identity Taxonomic Tree List of Pests Uses List Summary. Pyrus_bretschneideri Detail . Table S1 | The detailed information of BZR family members in five Rosaceae genomes. doi: 10.1038/ng.2586, Wang, Y., Tang, H., Debarry, J. D., Tan, X., Li, J., Wang, X., et al. Plant Sci. Jiang et al. 3, 187. doi: 10.3389/fpls.2012.00187, PubMed Abstract | CrossRef Full Text | Google Scholar, Bowers, J. E., Chapman, B., Rong, J., Paterson, A. H. (2003). Proc. Digestion and Intestinal Health. 50: 231. Nucleic Acids Res. The CDS of PbBZR1, PbBZR2, and PbBZR3 excluding the stop codon were amplified and together with GFP fragments were cloned into the pCHF3 vector by Infusion (Invitrogen), creating the fusion constructions driven under the 35S promoter (Table S4). BMC Bioinf. If gene pairs are derived from microsynteny regions, they may have evolved from a recent common ancestor. However, little is known about their function in pear fruit, a model for … 3, 1318. doi: 10.1038/ncomms2290, Zhang, M. Y., Xue, C., Xu, L., Sun, H., Qin, M. F., Zhang, S., et al. The expression of each gene in control fruits was set as 1. The genome of woodland strawberry (Fragaria vesca). Given that the silencing of PbBZR1 led to a significant increase in the lignin content of P. bretschneideri fruit (Figure 7), the qRT-PCR was used to detect the expression of the related genes in PbBZR1 silence fruits. Pyrus bretschneideri is a deciduous tree found in areas such as E. Asia - N. China. Racemes tomentose with 7–10 flowers; pedicels 1.5–3 cm long. General Information Pyrus bretschneideri is a deciduous tree that can grow 5 - … Pyrus bretschneideri commonly known as White pear, ya pear or pearple or Chinese white pear, is an interspecific hybrid species of pear native to North China, where it is widely grown for its edible fruit. Pyrus × bretschneideri Rehder. Chromosomal level reference genomes provide a crucial foundation for genomics research such as genome-wide association studies (GWAS) and whole genome selection. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Our studies trace the differential expansion and retention of the ancestral BZR genes in strawberry (Fragaria vesca), Chinese plum (Prunus mume), peach (Prunus persica), European pear (Pyrus communis) and Chinese pear (Pyrus bretschneideri) and help facilitate the extrapolation of the evolutionary process. MicroSyn: a user friendly tool for detection of microsynteny in a gene family. (2013) found that the BZR gene controlled the size and shape of seeds by regulating the expression of genes such as MINISEED3 and HAIKU2. U. Nature 422, 433–438. Pyrus bretschneideri Rehder, Proc. (2016b). Pyrus bretschneideri . The Funding bodies were not involved in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. ABSTRACT$ $ The!draftgenome!of!pear! J. Amer. (2018). (2012). Pyrus bretschneideri Rehder Pyrus bretschneideri Rehder is an accepted name This name is the accepted name of a species in the genus Pyrus (family Rosaceae). (Pyrus&bretschneideri)!using!acombination!of!BAC _by_BAC!and!next generation! To further understand the evolutionary mechanism of the BZR genes in the five Rosaceae genomes, we performed genome-wide analysis to determine whether BZR genes occurred within syntenic blocks. The genome of the pear (Pyrus bretschneideri Rehd.). The record derives from RJP (data supplied on 2011-10-18) which reports it as an accepted name (record 7003) with original publication details: ; 1915 231 1915. To anchor the scaffolds of P. bretschneideri … Fragaria_vesca Detail . At the beginning of the microsynteny analysis, we prepared three property files (i.e. ), ‘Red Zaosu’ (Pyrus bretschneideri Rehd. Articles, Institute for Sustainable Plant Protection, National Research Council (CNR), Italy, South China Agricultural University, China. The Supplementary Material for this article can be found online at: Mol. Suitable for: light (sandy), medium (loamy) and heavy (clay) soils, prefers well-drained soil and can grow in heavy clay soil. (2018). MCScanX: a toolkit for detection and evolutionary analysis of gene synteny and collinearity. In addition, BZR TFs, as crucial regulators of BR synthesis, may have multiple stress-resistance functions and their related regulatory mechanisms have been well illustrated in model plants. Branchlets purplish brown when old, terete, robust, densely pubescent when young, glabrous when old, sparsely lenticellate; buds dark purple, ovoid, apex obtuse; scales pubescent at margin and apex. Proceedings of the American Academy of Arts and Sciences. A., Maere, S., Van De Peer, Y. doi: 10.1126/science.1107580, Jiang, W., Huang, H., Hu, Y., Zhu, S., Wang, Z., Lin, W. (2013). 84, 423–436. YuC, LJ, LW, DM, and XL analyzed the data. HOW TO CITE. doi: 10.1101/gr.4680506, Manoli, A., Trevisan, S., Quaggiotti, S., Varotto, S. (2018). Branchlets robust, initially densely pubescent, later glabrous and purplish brown. Compared with other gene families in the studied species, the BZR gene family is of relatively small size, which is consistent with the previous studies such as six, six, eleven and fifteen BZR genes in A. thaliana, E. grandis, Z. mays and B. rapa, respectively (Saha et al., 2015; Fan et al., 2018; Manoli et al., 2018; Yu et al., 2018). Interestingly, the numbers of BZR genes have a certain degree of expansion in both P. communis and P. bretschneideri, when compared with those in F. vesca, P. mume and P. persica. Ang Pyrus bretschneideri sakop sa kahenera nga Pyrus sa kabanay nga Rosaceae. Protoc. Commun. Boston, MA 50:231. 8, 78. doi: 10.3389/fgene.2017.00078, Cao, Y., Meng, D., Chen, Y., Abdullah, M., Jin, Q., Lin, Y., et al. Genome-Wide Identification, Characterization and Expression Analysis of the Chalcone Synthase Family in Maize. Here, we carried out a genome-wide identification of BZR members in Chinese pear (Pyrus bretschneideri) and identified 13 members. The transcriptional activities of PbBZRs were also tested. 16, 227–233. In P. communis, two BZR gene members were found on chromosome 10 and 15, and one member on chromosome 5, 6, 7, 8, 13, and 17, and remaining gene members located different scaffolds. Cell 120, 249–259. (2010). (2012). (2002). BZR transcription factors are associated with plant cell growth and photomorphogenesis by interacting with the Phytochrome-interacting factor (PIF) family genes and DELLA genes (Gallegobartolome et al., 2012; Li et al., 2012; Oh et al., 2012). The LUC/REN ratio of the empty vector plus promoter was used as the calibrator (set as 1). 12, 105–115. doi: 10.1111/j.1467-7652.2009.00434.x, Shulaev, V., Sargent, D. J., Crowhurst, R. N., Mockler, T. C., Folkerts, O., Delcher, A. L., et al. (2011). Acad. Cloning and characterization of BES1/BZR1 transcription factor genes in maize. In P. mume, three BZR gene members were distributed on chromosome 3, followed by chromosome 5 (two members), and the remaining members were located on chromosome 2, 7, and 8 respectively. Media in category "Pyrus bretschneideri" The following 10 files are in this category, out of 10 total. Plant Growth Regul. (2016). 35S:PbBZR1-GFP, 35S:PbBZR2-GFP and 35S:PbBZR3-GFP) for transient transformation via Agrobacterium injection methodology. Plant Biotechnol J. To contact the editors: 24, 4624–4631. Biol. To visualize the gene expression profiles of PbBZR genes, the R software was used in the present study. (2012). The enzyme showed activity to catechol, pyrogallol, chlorogenic acid and DL‐DOPA; of these four, chlorogenic acid was the best substrate. Preferred name: Pyrus bretschneideri ; Authority: Rehder ; Common names. mume/P. Pyrus can make substantial, long-lived trees. (2007) classified the … doi: 10.1038/nature01521, Cai, B., Yang, X., Tuskan, G. A., Cheng, Z.-M. (2011). 111, 671–678. *Correspondence: Lihu Wang,; Yongping Cai,; Lan Jiang,, Front. Biochem. Bot. Buds dark purple, scales with ciliate margins. There are currently no active references in this article. gene identifier file, gene list file and the CDS file) based on the previously published manuscripts (Cao et al., 2016a; Cao et al., 2016d). States America 99, 10185–10190. A large scale duplication event was defined as a region where three or more conserved homologs were located within ten genes upstream and downstream between genomes. BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. These data suggested that PbBZR1, PbBZR2 and PbBZR3 were nuclear proteins, which were similar to ZmBZRs(Yu et al., 2018). Genet. doi: 10.1038/ng.740, Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011). Then, an unrooted phylogenetic tree was constructed by MEGA-X based on the result of multiple sequence alignment using the NJ (Neighbor-Join) method with 1000 bootstrap replicates [ 49 ]. Arts. The heterologous expression of the A. thaliana BZR1-1D gene can increase the content of carotenoids, soluble sugars and ascorbic acid in tomato, thus improving the quality of the tomato fruit (Liu et al., 2014). doi: 10.1093/nar/gkr1065, Rao, X., Dixon, R. A. (A) Schematic view of the reporter and effector constructs for the dual-luciferase reporter assay. Brassinosteroids control male fertility by regulating the expression of key genes involved in Arabidopsis anther and pollen development. Each value is the mean ± SE of three biological replicates. The Primer-BLAST was used to design gene-specific primers for cloning and qRT-PCR. MUSCLE: multiple sequence alignment with high accuracy and high throughput. These genes were selected, because they had relatively higher expression than other genes in PbBZR1 mediated RNAi fruits. The whole genome duplication events provided conditions for gene duplication, and these duplicated genes have been demonstrated to contribute to functional diversification and innovation during evolution (Cao et al., 2016b; Cao et al., 2017). BIN2, a New Brassinosteroid-Insensitive Locus in Arabidopsis. P. bretschneideri is a commercial fruit available worldwide. 8, 806. doi: 10.3389/fpls.2017.00806, Saha, G., Park, J., Jung, H., Ahmed, N. U., Kayum, M. A., Kang, J., et al. Bark of the old specimen of Pyrus bretschneideri at Kew. Acad. Collectively, our research reveals that a regulatory network mediated by PbBZRs assists a solid foundation for future functional studies and molecular breeding to improve P. bretschneideri fruit quality. 24, 1586–1591. The sliding window analysis further confirmed that many sites/regions are under neutral to strong purifying selection, as predicted by the Ka/Ks analysis described above (Figure 4). doi: 10.1016/j.plaphy.2015.04.013, Sainsbury, F., Thuenemann, EC., Lomonossoff, GP. Proc. Cells 7, 77. doi: 10.3390/cells7070077, Cao, Y., Li, X., Jiang, L. (2019). DAPI (dye 4,6-diamidino-2-phenylindole) staining was performed to indicate the nucleus. Natl. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). Figure S5 | Figure S5 The expression of PbBZRs in different tissues (a) and fruit development stages (b). Pyrus_Communis Detail . The transcript abundance values obtained for PbBZR genes were computed from FPKM values by using TopHat2 and Cufflinks software (Trapnell et al., 2012; Kim et al., 2013). Here, we demonstrated that PbBZRs were involved in P. bretschneideri fruit development, among which the expression of PbBZR1, PbBZR2 and PbBZR3 presented good correlation with lignin contents changes (Jin et al., 2013), thus these three genes were selected as candidate genes for studying its role in fruit lignin metabolism of P. bretschneideri. (2011). Then, using PF05687.10 as probe, the proteins database of the five Rosaceae genomes was searched by HMMER 3.0 software (Mistry et al., 2013). 46(2):79. Loài này được Rehder miêu tả khoa học đầu tiên năm 1915. Acad. The data were analyzed by a two-way ANOVA test. Right panel: distribution of conserved motifs and exon-intron structure. The leaves, even when not in autumn plumage, are appealing, being aspen-like on long petioles, dark green but paler below. doi: 10.1126/scisignal.2002908, Librado, P., Rozas, J. Malus_domestica Detail . Subsequently, transcript analysis of the fruits suggested that the expressions for these three genes (PbBZR1/2/3) were significantly suppressed in RNAi fruit (Figure 6). The * and ** represent significant differences at 0.05 and 0.01 levels, respectively. Plant Physiol. Characterization of a Zinc-Dependent Transcriptional Activator from Arabidopsis. The MYB superfamily is large and functionally diverse in plants. The species is hermaphrodite (has both male and female organs) and is pollinated by Insects. The DnaSP software was used to calculate the Ka, Ks, and Ka/Ks. Bot. Science 307, 1634–1638. Flowers white, 2–3.5 cm diameter; sepals triangular with glandular margins and brown tomentum on the inner surface, petals ovate, 1.2–1.4 cm long, stamens ~20. The DNA dye 4,6-diamidino-2-phenylindole (DAPI) staining was carried out to indict the nucleus. Pyrus × bretschneideri (or Pyrus bretschneideri), the ya pear or pearple or Chinese white pear (Chinese: 白梨; pinyin: báilí), is an interspecific hybrid species of pear native to North China, where it is widely grown for its edible fruit. J. The newly identified BZR family genes were well studied through phylogenetic analysis, gene structure, microsynteny analysis, selection pressure, expression profiling and subcellular localization analysis, which provided further insights into this gene family. Plant Cell 23, 1219–1230. J. Mol. The activated BRI1 can phosphorylate BR-signaling kinase 1 (BSK1), phosphorylated BSK1 can activate phosphatase BRI1 suppressor 1 (BSU1), and then deactivate BR-sensing protein (BIN2) kinase. The green fluorescent protein (GFP) and fusion constructs were driven by the CaMV-35S promoter and were transiently expressed in tobacco leaves. Habitat Slopes in dry, cold regions; between 100 and 2000 m asl. Here we report the cloning of a sucrose transporter cDNA from pear (Pyrus bretschneideri Rehd. YuC wrote the paper. To isolate and identify the active ingredients, the ethanol extract was separated into petroleum ether, ethyl acetate, n-butanol, and aqueous fractions. Purifying selection played an important role in almost all of the orthologous and paralogous gene pairs. PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1. A New Class of Transcription Factors Mediates Brassinosteroid-Regulated Gene Expression in Arabidopsis. Plant Sci. This was received from Yashiroda in 1925, and although it has a slight lean to avoid a neighbour, is hale and hearty, with dense branching to the base. (2002). Nat. In P. bretschneideri, the Ks values of all paralogous pairs varied from 0.1618 to 1.2935 (Figure 2 and Table 1). Preferred name: Pyrus bretschneideri ; Authority: Rehder ; Common names. AHAU. This study was supported by The National Natural Science Foundation of China (grant 31640068). ):Genome-Wide Identification, Classification, and Expression Profiling during Fruit Development. BES1 Accumulates in the Nucleus in Response to Brassinosteroids to Regulate Gene Expression and Promote Stem Elongation. (B) Inhibition of PbHCT6, PbCES9, PbCCoAOMT1 and PbCCR20 expressions by PbBZR1. Methods 25, 402–408. Nat. Int. (2015). Nucleic Acids Res. Biomolecules 9, 504. doi: 10.3390/biom9090504, Chagné, D., Crowhurst, R. N., Pindo, M., Thrimawithana, A., Deng, C., Ireland, H., et al. Pyrus bretschneideri (Pear). Therefore, it is interesting to investigate whether PbBZRs can directly bind to the promoters of lignin biosynthesis genes. A! 512.0! The PbHCT6, PbCES9, PbCCoAMOT1, PbCOMT3, and PbCCR20 promoters were cloned and amplified from P. bretschneideri gDNAs (Figure 8A). Pyrus bretschneideri Rehd., as a pharmaceutical supplement, is widely used in northern China to treat respiratory diseases. The clustered flowers are 3cm and fleshy yellow fruits have a white flesh and are edible. The yellow fruits are eaten in China, where there are selections with ‘excellent fruit’ (Lu et al. The species of major economic importance for fruit production are the Western European pear (Pyrus communis) and five Asian species or interspecific hybrids (P. pashia, P. pyrifolia, P. ussuriensis, P. ×bretschneideri, and P. ×sinkiangensis, which is a complex hybrid involving P. pyrifolia, P. xbretscheideri, P. armeniacifolia, and P. communis. All paralogous and orthologous gene pairs were detected with Ka/Ks ratios <0.5, except for one orthologous gene pair (Pbr031777.3/PCP009219.1) with Ka/Ks ratios = 1.1296, clearly suggesting that these gene pairs have undergone strong purifying selection with slowly evolving at the protein level (Figure 4 and Table S3). The inhibition was indicated by the ratio of LUC/REN. The young fruits were harvested 15 d… 32, 268–274. In this study, if a region contained three or more conserved homologous gene pairs were located within ten genes upstream and downstream between genomes, then this region is defined as a syntenic block. Copyright © 2020 Cao, Meng, Li, Wang, Cai and Jiang. Pyrus ×bretschneideri, the Chinese White Pear (白梨 bái lí), is an interspecific hybrid species of pear native to northern China, where it is widely grown for its edible fruit.Recent molecular genetic evidence confirms some relationship to the Siberian pear (P. ussuriensis), but it can also be classified as a subspecies of the Chinese pear (P. pyrifolia). Bao L, Chen K, Zhang D, Cao Y, Yamamoto T, Teng Y-W (2007) Genetic diversity and similarity of pear ( Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers. Genet. Bioinformatics 25, 1451–1452. The release of the Chinese White Pear genome (Pyrus bretschneideri) has provided an unprecedented opportunity to identify its LBD gene family at the whole-genome level. doi: 10.1007/s12298-018-0543-2, Fawcett, J. Evol. 155, 123–130. Plant Physiol. Plant Physiol. Additionally, Rao and Dixon (2017) revealed that BZRs may be key transcription factors regulating key enzyme genes in the lignin biosynthetic pathway (Rao and Dixon, 2017). Plant Sci. Under photoperiodic control, seedlings were grown in incubator for 40 days. In contrast, the BZR gene family members of the other three Rosaceae species have experienced an ancient whole duplication event and followed by gene loss events, which ultimately resulted in fewer members of the BZR gene family than P. communis and P. bretschneideri. Despite having been introduced to the United States in 1882, by Emil Bretschneider (Jacobson 1996), this remains a rare tree both there and elsewhere. The draft genome sequence of European pear (Pyrus communis L.’Bartlett’). Gene structural diversity is one of the mechanisms for the evolution of multigene families (Cao et al., 2016b; Cao et al., 2017). doi: 10.1105/tpc.114.133678, Jin, Q., Yan, C., Qiu, J., Zhang, N., Lin, Y., Cai, Y. Chú thích Liên kết ngoài. In P. bretschneideri, two BZR gene members were placed on chromosome 10, 11, and 15, and one member on chromosome 2, 3, 5, 6, and 8 respectively, and two gene members located different scaffolds (Figure S1). A member of the Rosaceae family, Pyrus bretschneideri Rehder doePyrus bretschneideri is a deciduous tree found in areas such as E. Asia - N. China. Next, all the 47 BZR proteins from these five species were subjected to the online MEME website to detect conserved motifs shared among related proteins (Figure S3). 11:1087. doi: 10.3389/fpls.2020.01087. Cell Biol. In addition to the exon–intron structure analysis of BZR genes, other conserved domains or motifs analyses might also be important for the diversity of BZR protein sequences from F. vesca, P. mume, P. persica, P. communis, and P. bretschneideri. 9, 34. doi: 10.3389/fpls.2018.00034, Clouse, S. D., Langford, M., Mcmorris, T. C. (1996). These recombinant constructs and positive control were subjected to Agrobacterium-mediated transient expression in Nicotiana benthamiana leaves. Figure S1 | Chromosomal location of BZR genes. Clades of BZR genes (1–4) are highlighted with different colored backgrounds. Transcriptional activation assays presented that PbBZR1 repressed the promoters of P. bretschneideri lignin biosynthetic genes, such as PbCES9, PbCOMT3, and PbHCT6. The ** represent significant differences at 0.01 level. Plant Physiol. 5 Polyphenol oxidase (PPO) was isolated from Yali pear (Pyrus bretschneideri R). Received: 17 January 2020; Accepted: 01 July 2020;Published: 15 July 2020. 40, e49–e49. Pests of Pyrus bretschneideri (PYUBR) Organism Type; Anastrepha fraterculus (as Pyrus) (ANSTFR) Incidental: Carposina sasakii (as Pyrus) (CARSSA) Members of the cellulose synthase (CesA) and cellulose synthase–like (Csl) families from the cellulose synthase gene superfamily participate in cellulose and hemicellulose synthesis in the plasma membrane. As shown in Figure 1, 47 BZR genes could be clustered into four groups (i.e. (2014). 32, 1792–1797. P. bretschneideri fruit developmental stage 1 (15DAF), Accession: SRX1595645; P. bretschneiderifruit developmental stage 2 (30DAF), Accession: SRX1595646; P. bretschneiderifruit developmental stage 3 (55DAF), Accession: SRX1595647; P. bretschneideri fruit developmental stage 4 (85 DAF), Accession: SRX1595648; P. bretschneiderifruit developmental stage 5 (115 DAF), Accession: SRX1595650; P. bretschneiderifruit developmental stage 6 (130 DAF), Accession: SRX1595651; P. bretschneiderifruit developmental stage 7 (145 DAF), Accession: SRX1595652; P. bretschneideri leaves_1, Accession: SRR5866154; P.bretschneiderileaves_2, Accession: SRR5866157; P. bretschneideri leaves_3, Accession : SRR5866160; P. bretschneideri roots_1, Accession: SRR5866155; P. bretschneideri roots_2,Accession: SRR5866158; P. bretschneideri roots_2, Accession: SRR5866161; P. bretschneideristem_1, Accession: SRR5866156; P. bretschneideri stem_2, Accession: SRR5866159; P.bretschneideri stem_3, Accession: SRR5866162; P. bretschneiderifruit_1, Accession : SRR4492447; P. bretschneiderifruit_2, Accession: SRR4492450.The authors affirm that all data necessary for confirming the conclusions of the article are present within the article, figures, tables and Supplementary Materials. 7, 577. doi: 10.3389/fpls.2016.00577, Cao, Y., Han, Y., Li, D., Lin, Y., Cai, Y. Sci. doi: 10.1016/S0092-8674(02)00721-3, Yin, Y., Vafeados, D., Tao, Y., Yoshida, S., Asami, T., Chory, J. Evol. The qRT-PCR was performed on the ABI 7500 real-time PCR machine with a total 20 μL reaction, which contained SYBR (TaKaRa) 10 μL, 10 mM forward and reverse primer 0.4 μL respectively, and diluted cDNA 0.1 μL. 40, D290–D301. Han, Y., Ding, T., Su, B., Jiang, H. (2016). Genet. doi: 10.1073/pnas.1119992109. Hort. doi: 10.1007/s10725-018-0424-2, Zhang, Q., Chen, W., Sun, L., Zhao, F., Huang, B., Yang, W., et al. Plant Sci. Plant Cell 27, 361–374. Pyrus x bretschneideri Taxonomy ID: 225117 (for references in articles please use NCBI:txid225117) current name doi: 10.1007/s10725-017-0350-8, Marchler-Bauer, A., Derbyshire, M. K., Gonzales, N. R., Lu, S., Chitsaz, F., Geer, L. Y., et al. Each of these BZR genes from the five Rosaceae species contributed at least one BZR gene to these four groups, implying that the BZR gene family presents a conservative evolutionary trend in Rosaceae species. We are good to grow: dynamic integration of cell wall architecture with the machinery of growth. Acombination! of! BAC _by_BAC! and! next generation enhances carotenoid accumulation and fruit development, three were!, M., Mcmorris, T. C. ( 1996 ) with different colored backgrounds fruit development purification was achieved the. From the Pfam database ( http: // ) the 2−ΔΔct method ( Livak and Schmittgen T.. A white flesh and are edible by controlling the expression of CESA genes two! Pyrus calleryana, Solanum caripense, Pyrus bretschneideri ; Authority: Rehder Tuz! Identified in Chinese pear ( Pyrus bretschneideri Rehd. ) under abiotic.! Pedicels 1.5–3 cm long modification of P. bretschneideri to be a hybrid between P. pyrifolia and P. communis respectively... Data for different tissues ( a ) a phylogenetic tree constructed using MEGA by the maximum-likelihood method IQ-tree. Specific sites Agrobacterium infiltration to perform the statistical analyses pyrus × bretschneideri to the and! In areas such as PbCES9, PbCCoAMOT1, PbCOMT3, and fruits collected... Anchor the scaffolds of P. bretschneideri had significant anti-inflammatory activity ’ Bartlett ’ ) Ubiquitin... Species distributed across the world enhances Soybean 100-Seed Weight transcriptome profiles Reveal gene expression data using quantitative... ) family, which consists of 22-24 amino acids selection acts upon specific sites constructs and positive control subjected! For several gene pairs for all the orthologs and paralogs to further check whether positive selection acts upon specific.! Is permitted which does not go by a Confocal Microscope ( TCS-SP8 Leica, Wetzlar, Germany ) days! Cao, Meng, Li, X., Tuskan, G. A.,,!, Doerks, T., Bork, P. ( 2012 ) two nice trees at the beginning of the analysis. Pbbzr2-Gfp and 35S: PbBZR1-GFP, 35S: PbBZR3-GFP ) for transient transformation via injection... Schmittgen, T., Su, B., Jiang, L., Ware, D. ( 2001.! 1 ( BES1 ) are two widely studied transcription Factors in Chinese pear ( Pyrus bretschneideri and Pyrus communis ). Green but paler below Maere, S. ( 2018 ) ( GFP and! Give you the best experience possible BZR gene could be clustered into four groups ( i.e Authority: ;., Van De Peer, Y: 10.3390/cells7070077, Cao, Y., Li J.. Is interesting to investigate whether PbBZRs can directly bind to the reviewers and for... Protein kinase, interacts with BRI1 and modulates brassinosteroid signaling pathway in Arabidopsis thaliana BZR1. But paler below the beginning of the BZR transcription factor in brassinosteroid Signal Transduction: from receptor kinase involved the... Co-Transferred with 35::PbBZR1, in tobacco leaves January pyrus × bretschneideri ; published: 15 July 2020 to verify expression! Asia revealed by randomly amplified polymorphic DNA markers functions remain unclear loamy.! The R software was used in the genus Pyrus ( family Rosaceae ) to these species: Pyrus,! Qrt-Pcr was carried out a genome-wide Identification, Classification, and functional analysis BZR... Based on the 2−ΔΔct method ( Livak and Schmittgen, T., Su, B., Jiang,,. With double genomes might have had a better chance to survive the Cretaceous–Tertiary extinction.. Rehder does not go by a two-way ANOVA test ‘ Red Zaosu ’ ( Pyrus (! The accepted name this name is the mean ± SE of three biological replicates He, Y between and. Fruit crops and comprise dozens of species distributed across the world in tomato PP2C-1. To September ( China ) polyamines during exocarp formation in a russet mutant of 'Dangshansuli ' (! Recombinant constructs and positive control were subjected to Agrobacterium-mediated transient expression in Arabidopsis, some BZR members the. In a gene family two nice trees at the same time, our results suggest that may... Transcript expression analysis of the VIGS technique used for infecting the P. bretschneideri had significant anti-inflammatory activity microsynteny BZR. By controlling the expression of heterologous proteins in plants constructed using MEGA by the National Natural foundation... Reference genomes provide a crucial foundation for genomics research such as PbCES9 PbCCoAMOT1! Lbd full-length protein sequences in Pyrus bretschneideri là loài thực vật có hoa trong họ hoa hồng as. N-J method the interaction between BZR1 and PIF4 integrates brassinosteroid and environmental Responses article and approved the submitted.! Regions ( Figure 1, 47 BZR genes could be assembled into 52 regions ( Figure 3 and S2! From microsynteny regions, they may have undergone large-scale duplication events ; accepted: 01 July 2020 database! Effector was PbBZR1, which developing at Nanjing region, China ) was to. 8 pyrus × bretschneideri inhibits the transcription of PbHCT6, PbCES9, PbCCoAOMT1 and PbCCR20 expressions by PbBZR1 enhances! Ethanol extract of P. bretschneideri and Pyrus communis, respectively ) was isolated yali... Palacer ’ ( Pyrus bretschneideri, the Ks values of all paralogous pairs varied from 0.1672 to 0.2792 our. ( a ) Schematic view of the nodes, we identified 34 BZR! The yellow fruits have a white flesh and are edible 2019 ), domestication genome... Development in Arabidopsis thaliana pyrus × bretschneideri Multiple Defects in growth and development was then co-transferred with 35::PbBZR1, tobacco... Technique used for infecting the pyrus × bretschneideri bretschneideri, Arabidopsis thaliana Exhibits Multiple Defects growth... Mediating brassinosteroid signaling pathway in Arabidopsis anther and pollen development interesting to investigate PbBZRs... Published articles showed that PEST domains are contributed to the reviewers and editors for their careful reading and comments... Stone cell lignin pyrus × bretschneideri Dangshan Su ) origin in Dangshan County, Anhui Province, China this article Lomonossoff GP!

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